With its linear and reflector modes of operation it addresses a wide range of applications. Revisionedecember2015 instructionsforuse brukerguidetomaldisample preparation careproductsaredesignedtosupportourworldwidecustomerswithhighqualityconsumables. Nmr user manuals and technical documentation in pdf format. I have tried and will continue to try to use the bruker manual, but so far i havent been lucky yet. Access training videos, machine manuals, software updates and more. Full idast solution integrating bd phoenix and bruker microflex via bd epicenter, optionally using the bd phoenix ap. The microflex ltsh is the affordable entrylevel member of the flex line of mass spectrometers that provide quick and reliable results from the smallest sample amounts.
Bruker nanoelute is designed with years of proteomics knowhow and nanouhplc expertise to deliver robust and reliable operation from the. Bruker microflex series innovation mass spectrometry systems by bruker corporation. The microflex offers high resolution reflectron mode for compounds less than 5,000 da and low resolution linear mode for compounds greater than 5,000 da. Bruker microflex malditof open access the microflex lrf is specialized for protein, peptide, and polymer analysis. Realtime comparative evaluation of biomerieux vitek ms versus bruker microflex ms, two matrixassisted laser desorptionionization timeofflight mass spectrometry systems, for identification of clinically significant bacteria. Vitek ms had a relatively higher noidentification rate compared to bruker microflex lt in the coryneforms and. Scion 456gc bruker daltonics pdf catalogs technical. Expert users and beginners can quickly and confidently tap into the analytical power and flexibility of brukers flex series malditof systems. Our breakthough design offers a worryfree thermal flow switch that does not require any user adjustments or calibration. Autoflex basic user manual last updated august 26, 2009. This equipment may be connected to other machines that have rotating parts or parts that are.
Get support and info on your specific machine, presorted for you. The bruker maldi biotyper system is an industryleading microbiology system used for rapid microbial identification. English, german, french, spanish, italian, portuguese, cyrillic, kanji, chinese standard and traditional. Bruker contents 1malditargetplatetypes 3 2riskandsafetyinformation 4 3howtocleanmalditargetplates 4 4maldisamplepreparationprotocols 5 4. Documentation on some of these common hardware and software components is available in the users manuals for the d8 family of instruments. A user evaluation was conducted to qualitatively assess technologist impressions of the 2 platforms based on specific metrics related to suitability of the instruments in the current workflow. Bruker autoflex lrf maldi tof matrixassisted laser desorption ionization is a soft ionization technique. Bruker microflex maldi tof mass spec available at the lab world group a seller of used lab equipment. This malditof instrument can be operated in a linear mode for high mass accuracy characterization, or in a reflector mode to achieve enhanced resolution.
View and download abb microflex e150 user manual online. Performances and reliability of bruker microflex lt and vitek ms malditof mass spectrometry systems for the identification of clinical microorganisms. Direct method sample is applied to target plate toothpick pipette tip matrix is overlaid on sample. Indicates the off position of the main power switch. Evaluation of the bruker maldi biotyper for identification.
Comparison of the vitek ms and bruker microflex lt malditof. The microflex takes advantage of bruker daltonics long standing expertise in providing the highest performance mass spectrometers. The microflex ltsh malditof mass spectrometer with n2 laser at 60 hz repetition rate. The spectra were imported into the integrated maldi biotyper software version 3. Malditof bruker ms bms measurements were done with a microflex lt mass spectrometer bruker daltonics using flexcontrol software version 3. Direct instrument interface between bd epicenter and the bruker maldi biotyper. Jun 02, 2017 bruker nanoelute is designed with years of proteomics knowhow and nanouhplc expertise to deliver robust and reliable operation from the moment of installation through years of omics knowledge.
Malditof is a rapid, simple, inexpensive, userfriendly and highthroughput proteomic technique for. Bruker 7tsolarix icr ftms mass spectrometry center. This video is a tutorial on how to clean the bruker microflex in the dorrestein lab. Microflex e150 quick installation guide lt0307 safety manual. We usually use fleximaging software but it does not seem to be able to make movies. Bruker daltonik gmbh ultraflex iii mass spectrometer 1 ultraflex iii mass spectrometer the ultraflex iii is a maldi matrix assisted laser desorptionionization tandem mass spectrometer specially designed for automated ms and msms high throughput.
Brukerdaltonikgmbh tableofcontents tableofcontents legalandregulatorynotices 2 contact 4 tableofcontents 5 1introduction 9 2gettingstarted 11 2. Vitek ms had a relatively higher noidentification rate compared to bruker microflex lt in the coryneforms and other grampositive bacilli group table 5. Pdf performances and reliability of bruker microflex lt and. Indicates the on position of the main power switch. The microflex ltsh is a compact linearmode benchtop malditof system designed as an easytouse, but high performance solution for basic applications. With this technology, the lab has expanded identification capabilities to other organism groups and added direct presumptive identifications of organisms from positive blood cultures the same day the aerobic andor anaerobic blood culture bottles become positive. Explore the latest products and resources related to your industry. Technological innovations, prominent manufacturers and popular equipment all in one place. Bruker microflex series innovative mass spectrometry. Brukers microflex malditof system has more than met the labs expectations. How comarketing agreement between bd and bruker daltonics. Machine operation calibrate using flex control software. Together with the dxm microscan walkaway system, the bruker maldi biotyper can provide a rapid and accurate solution for id and ast testing. In addition to the user manual, instructions concerning labor protection laws, operator regulations, tools and supplies must be available and adhered to.
Standard features boiler or condensate conductivity input. Improper operation or programming of the drive may cause violent motion of the motor and driven equipment. Determine molecular weight range and polydispersity as well as chemical nature of repeat units. July 29, 2008 topics covered sample requirements instrument overview technical general instrument use instructions calibration discussion tips and tricks software reference at end of powerpoint file will not be covered in this session. Overview microflex series highest performance benchtop. This instrument is available for selfoperation after training. Free download ebooks this process also results in network interruption to the virtual machines. Bruker daltonik gmbh introduction microflex user manual, version 1. Drive disable, motor disconnect, motor brake and other means should be used as appropriate. How brukers user manuals help you to successfully apply our advanced software and use our hardware. Direct method sample is applied to target plate toothpick pipette tip.
The microflex lt benefits from bruker daltonics patented anchorchip technology which provides exactlypositioned samples on the malditarget for robust automatic measurements, as well as a sensitivity boost by up to two orders of magnitude. Safe torque off sto function for microflex e150 drives lt03 microflex e150 wall chart lt0296 microflex e150 flyer 3aua0000097609 microflex e150 ce certificate 3axd0409551 microflex e150 tuv certificate de00043100. The microflex ltsh is a true benchtop solution taking up less than 1 meter 40 of counter space to provide flexibility in meeting laboratory needs. Target plate loaded moves to position pulls vacuum. Run log file stored with the chromatogram when using compasscds local display. Our lab uses a bruker microflex malditof for imaging tissue samples and we wanted to make a movie of the data like in biomapexport image as.
Bruker biflex iii malditof user training jonathan a. The stop input to this equipment should not be used as the single means of achieving a safety critical stop. A side by side comparison of bruker biotyper and vitek ms. Each of our systems have been tested and backed with our standard warranty. However, consequently, both systems correctly identified 97% of routine clinical isolates making them reliable tools for clinical microbiology. The microflex offers high resolution reflectron mode for compounds less than 5,000 da and low resolution linear. It is also capable of tandem maldims for peptide sequencing and protein identification. In just a few steps, the simpletouse software guides users.
Whether it is biomarker discovery, oligonucleotide, small molecule or polymer analysis, or even the innovative fast an. The sample is mixed with a matrix small organic molecule that absorbs at the lasers wavelength in a common solution. July 29, 2008 topics covered sample requirements instrument overview technical general instrument use instructions calibration discussion tips and tricks software reference at end of powerpoint file will not be. Brukerdaltonikgmbh contact limitationsonuse forresearchuseonlyruo. The system is open and can be easily expanded by specific requests of the user including data for id of bacteria, yeasts and fungi.
Bruker maldi biotyper microbiology systems beckman coulter. Troubleshoot equipment issues or search or faqs for answers. The unique design of the microscout ion source and the gridless reflectron give the microflex superior resolution, as well as excellent mass accuracy and outstanding sensitivity. Designed for applications in need of a short time to result, the microflex smart ls features a fast solidstate bruker smartbeam laser and high performance vacuum system. Hyperlinkdisclaimer brukerdaltonikgmbhmakesnoexpresswarranty. Dec 11, 2014 this video is a tutorial on how to clean the bruker microflex in the dorrestein lab. The prominent microflex is a powerful controller integrating builtin diagnostics with realtime monitoring in a compact design. When the following prompt appears, just click on the logon button without changing anything. Of the microbiology technologists working at the time of study, 3549 71. Daltonik gmbh part numbers are generally listed in parentheses after the name of the part. Instructions for use bruker guide to maldi sample preparation. Abb motion control users manual microflex e150 servo drive. Microflex ltsh from bruker daltonics selectscience. Mass range up to 300,000 da up to 10,000 da in reflectron mode workhorse instrument for analysis of synthetic polymers.
Bruker microflex lt, however, confidently acts and reports single species, which can provide an advantage in research studies as well as in analyses of environmental, food, and industrial samples. Maldi biotyper integrates a readytouse reference library comprising thousands of individual strains and is growing continuously. Matrixassisted laser desorption ionizationtime of flight mass spectrometry malditof ms has emerged as a rapid, highly accurate, and costeffective method for routine identification of a wide range of microorganisms. The maldi can be operated in reflectron mode for highresolution mass analysis of peptide and lower molecular weight polymers, and in linear mode for analysis of large molecules e. It is easy to use with intuitive operations that are ideal for nonexpert users and busy multiinstrument labs. Autoflex basic user manual indiana university bloomington. On the other hand, one myroides odoratimimus isolate could only be identified to genus level by bruker microflex lt, whereas it was identified to species level by vitek ms. The microflex lt sh malditof mass spectrometer with n2 laser at 60 hz repetition rate. Comparison of the vitek ms and bruker microflex lt maldi. Introduction apex2 user manual 1 2 m86e00078 bruker products.
See section document library on the internet on the inside of. Bruker autoflex lrf maldi tof mass spectrometry research. Maldi is ideal for polymers, peptides, intact proteins and a wide variety of compounds. Malditoftof brukers flex series for quick and reliable results. For more information, see the relevant user manual or instructions for. Performances and reliability of bruker microflex lt and. Bruker daltonics is continually improving its products and reserves the right to change specifications without notice. We have great deals on used lcms and mass spectrometers from sciex, thermo scientific, agilent, bruker, shimadzu and more. Performances and reliability of bruker microflex lt and vitek.
List of related manuals you can find manuals and other produc t documents in pdf format on the internet. The acquisition software will then finish loading and initializing the instrument. We carried out a side by side comparative evaluation of the performance of bruker biotyper versus vitek ms for identification of a large and diverse collection of. Bruker daltonik gmbh table of changes version date changes remarks 1. The acquisition and analysis of mass spectra were performed by a microflex lt mass spectrometer bruker daltonik using the maldi biotyper software package version 3. Other members of this new generation of instruments include the d8 advance and d8 discover, and the d8 gadds systems for general diffraction.